Recombinant Protein A No antibody contaminant. No antibody affinity column purification. 5-Recombinant protein A.rar SOURCE E.Coli, HPLC purified. SEQUENCE Protein A mutant of Staphylococcus aureus MOL.WEIGHT 25 kDa FORM Lyophilized powder. Solution：50mg/ml in deionized water or PBS. PH STABLE RANGE pH 1-13 STORAGE CONDITION 2℃~8℃. APPLICATION The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form. Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests. PRODUCT SPECIFICATION Test/Method Specification Bioburden No organisms detected. 12% SDS-PAGE Single major band Coomassie Stain 25kD UV Spectrum E270/250≥1.2,E0.1% at 270nm=0.14-0.20 UV spectrometry No DNA or RNA detected Triton Content No Triton contaminant.
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.

CAS NO:91932-65-9

EC NO:295-242-9

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Synonyms:;Staphylococcal Protein A;CCRIS 4112;Protein A;Recombinant Protein A;SPA;

Molecular Structure: