Overview: Biotin, also known as Vitamin H or Coenzyme R, is a water-soluble vitamin that belongs to the Vitamin B family, specifically B7. It is an essential substance for the synthesis of Vitamin C and is indispensable for the normal metabolism of fats and proteins. It is a necessary nutrient for maintaining natural growth, development, and normal bodily functions in humans. This kit uses a microbiological method to detect the total amount of biotin (both added and naturally occurring) in infant formula. After incubating at 37°C in the dark for 23-24 hours, the turbidity of the samples is measured using a microplate reader at 620nm-630nm. By comparing the sample turbidity with the standard curve, the biotin content in the sample can be quantitatively determined.

Principle: Biotin is an essential nutrient for the growth of Lactiplantibacillus plantarum. The culture medium and processed sample extract are added to microplate wells coated with the bacterial strain. After incubation, the transmittance (or absorbance value) is measured. Within a certain detection range, the biotin content in the sample can be calculated based on the standard curve of biotin concentration versus transmittance (or absorbance value).